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Anti-tGFP Tag Antibody

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Specifications Citations (5) Related Products Product Documents
SKU Description Datasheet Price Availability  
TA150041 Mouse monoclonal turboGFP antibody, clone 2H8, 100ul $248 In Stock Add to Shopping Cart

Specification

Product NameMouse monoclonal turboGFP antibody, clone 2H8
Clone Nameclone 2H8
IsoTypeIgG2b
Concentration1mg/ml
ImmunogenAnti-tGFP monoclonal antibody was produced by immunizing mice with purified tGFP protein expressed in HEK293T cell.
Host SpeciesMouse
FormulationPBS (pH7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide
ReactivitytGFP
Validated ApplicationWB, IF, IP
Suggested DilutionWB: 1:2000, IF: 1:100
Predicted Size26KDa
Storage/StabilityStable for 1 year at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing.
WB Image
Immunoblot analysis of randomly picked 7 different tGFP tagged over-expression lysates with 2H8 anti-tGFP monoclonal antibody (TA150041) at 1:2000 dilution.
IF Image
Cos7 cells transiently transfected with turboGFP tagged BID ORF cDNA clone (RC100007) for mitochondria marking were immuno-stained with OriGene 2H8 anti-turboGFP antibody (TA150041, 1:100), and then stained red with an Alexa-568 conjugated secondary antibody (1:1000).
IF Image
Cos7 cells transiently transfected with turboGFP tagged LAMP1 ORF cDNA clone (RC100016) for lysosome marking were immuno-stained with OriGene 2H8 anti-turboGFP antibody (TA150041, 1:100), and then stained red with an Alexa-568 conjugated secondary antibody (1:1000).
IP Image
Immunoprecipitation (IP) of recombinant tGFP fusion protein from overexpression lysate made with HEK293T cells transfected by plasmid expressing tGFP-DDK. The immunoprecipitated tGFP fusion protein was analyzed by immunoblot with rabbit anti-DDK polyclonal antibody. (Left lane: Negative control, IP without adding TA150041)

 

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