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Clone OTI4C5, Anti-DDK Agarose beads
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|Product Name||Clone OTI4C5, Anti-DDK Agarose beads|
|Clone Name||clone OTI4C5 (formerly 4C5)|
|Immunogen||Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide (DYKDDDDK) coupled to KLH|
|Formulation||TBS (pH7.4) containing 50% Glycerol, 0.02% NaN3|
|Reactivity||DDK (FLAG tag): DYKDDDDK|
|Validated Application||IP, Protein Purification|
|Suggested Dilution||IP: see datasheet|
|Storage/Stability||Store at -20ºC. Stable for at least 1 year from date of shipment.|
Immunoprecipitation was performed with HEK293T cell lysates of expressing Entry vector (control, Cat# PS100001) or expressing DDK-tagged PON3 (Cat# LY400339). Anti-DDK 4C5-agarose beads were incubated with control lysate or LY400339 overnight at 4C respectively. After washing three times with lysate buffer, 20uL 4x SDS sample buffer was applied. The samples were boiled at 95C for 10min. The supernatant was loaded to the SDS-PAGE gel after discarded the beads by centrifuging. After transferring to the membrane, the Western blot assay was applied with HRP-conjugated anti-DDK antibody (Cat# TA150030). Lane 1: control lysate; Lane 2: DDK-tagged PON3 (LY400339); Lane 3: anti-DDK 4C5 agarose beads (TA150037) 50uL with control lysate; Lane 4: anti-DDK 4C5 agarose beads (TA150037) 50uL with LY400339.