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Clone OTI4C5, Anti-DDK (FLAG) Agarose beads


Specifications Citations (3) Customer Reviews Product Documents
SKU Description Datasheet Price Availability  
TA150037 Clone OTI4C5, Anti-DDK (FLAG) Agarose beads $368 In Stock Add to Shopping Cart
Conjugation is available for this antibody: choose conjugation type
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Product NameClone OTI4C5, Anti-DDK (FLAG) Agarose beads
See all DDK (FLAG) Antibodies >>
Clone Nameclone OTI4C5 (formerly 4C5)
ImmunogenAnti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide (DYKDDDDK) coupled to KLH
Host SpeciesMouse
FormulationTBS (pH7.4) containing 50% Glycerol, 0.02% NaN3
ReactivityDDK (FLAG tag): DYKDDDDK
Validated ApplicationIP, Protein Purification
Suggested DilutionIP: see datasheet
Predicted Size
Storage/StabilityStore at -20ºC. Stable for at least 1 year from date of shipment.
Note This antibody doesn't recognize N- terminus DDK tag. Please use TA180144 for the detection of DDK tag at any location in Western blotting.
IP Image
Immunoprecipitation was performed with HEK293T cell lysates of expressing Entry vector (control, Cat# PS100001) or expressing DDK-tagged PON3 (Cat# LY400339). Anti-DDK 4C5-agarose beads were incubated with control lysate or LY400339 overnight at 4C respectively. After washing three times with lysate buffer, 20uL 4x SDS sample buffer was applied. The samples were boiled at 95C for 10min. The supernatant was loaded to the SDS-PAGE gel after discarded the beads by centrifuging. After transferring to the membrane, the Western blot assay was applied with HRP-conjugated anti-DDK antibody (Cat# TA150030). Lane 1: control lysate; Lane 2: DDK-tagged PON3 (LY400339); Lane 3: anti-DDK 4C5 agarose beads (TA150037) 50uL with control lysate; Lane 4: anti-DDK 4C5 agarose beads (TA150037) 50uL with LY400339.


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