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Anti-tYFP Tag Antibody
|Product Name||Mouse monoclonal turboYFP antibody, clone 10F11|
|Clone Name||clone 10F11|
|Concentration||0.5 - 1 mg/ml|
|Immunogen||Full length tYFP protein expressed in HEK293T cells|
|Formulation||PBS (pH 7.3) containing 1%BSA, 50% glycerol and 0.02% sodium azide|
|Validated Application||WB, IF, FC|
|Suggested Dilution||WB: 1:2000, IF: 1:50, FC: 1:400|
|Storage/Stability||Stable for 1 year at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing.|
HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY tYFP (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-tYFP (TA150027).
Anti-tYFP mouse monoclonal antibody (TA150027) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY tYFP (pCMV6-tYFP).
HEK293T cells transfected with either pCMV6-ENTRY tYFP (pCMV6-tYFP) (Red) or empty vector control plasmid (Blue) were immunostained with anti-tYFP mouse monoclonal antibody (TA150027), and then analyzed by flow cytometry.