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Anti-tGFP Tag Antibody
Specification
| Product Name | Mouse monoclonal tGFP antibody, DyLight 594 conjugated |
| Clone Name | clone 2H8 |
| IsoType | IgG2b |
| Concentration | 0.5mg/ml |
| Immunogen | Anti-tGFP monoclonal antibody was produced by immunizing mice with purified tGFP protein expressed in HEK293T cell. |
| Host Species | Mouse |
| Formulation | TBS (pH7.4) containing 0.1% BSA, 0.05% NaN3 and 50% Glycerol; 100uL/each with 500ug/ml |
| Reactivity | tGFP |
| Validated Application | IF, FC |
| Suggested Dilution | |
| Predicted Size | 26KDa |
| Storage/Stability | Stable for 1 year at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing. |
IF Image
Hela cells transiently transfected with Paxillin-GFP (RG225997) were detached and seeded onto 0.2% gelatin coated coverslip. After 24 hours incubation, the cells were fixed with 4% formaldehyde for 30 min, permeabilized with 0.1% trixon-X100, and then stained with Dylight594-labeled tGFP antibodies (TA150024) and DAPI for nucleus counter staining. (a: Paxillin-GFP; b: anti-tGFP-DyLight594; c: Merged)
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