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Clone OTI4C5, Anti-DDK monoclonal antibody, DyLight 488
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|Product Name||Clone OTI4C5, Anti-DDK monoclonal antibody, DyLight 488 |
|Clone Name||clone OTI4C5 (formerly 4C5)|
|Immunogen||Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide (DYKDDDDK) coupled to KLH|
|Formulation||TBS (pH7.4) containing 0.1% BSA and 0.05% NaN3|
|Reactivity||DDK (FLAG tag): DYKDDDDK|
|Validated Application||IF, FC|
|Suggested Dilution||FC: 1:50, IF: 1:50|
|Storage/Stability||Store at -20ºC, stable for at least three months from date of shipment|
CHO cells were tranfected with BAF57-Myc/DDK plasmid (OriGene Cat# RC209444). The cells were fixed with 2% formaldehyde for 20 min, and permeabilized with 0.1% saponin in PBS for 10 min. The cells were then incubated with DyLight488-labeled DDK antibodies diluted 1:50 in PBS/0.1% saponin for 20 min. The cells were then washed and observed under a fluorescent microscope. Please note the nucleus staining pattern for BAF57.
HEK293T cells were transfected with PON3 cDNA (Cat# RC210019). After 48 hours, the cells were detached, fixed with 2% formaldehyde for 30 min, and permeabilized with 0.1% trixon-X100. The cells were then stained with DyLight488-conjugated mouse IgG1 (black line) or DyLight488- conjugated DDK antibodies diluted at 1:100 (red line).