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Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, DyLight 488


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SKU Description Datasheet Price Availability  
TA150021 Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, DyLight 488 $318 In Stock Add to Shopping Cart
Conjugation is available for this antibody: choose conjugation type
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Product NameClone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, DyLight 488
See all DDK (FLAG) Antibodies >>
Clone Nameclone OTI4C5 (formerly 4C5)
ImmunogenAnti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide (DYKDDDDK) coupled to KLH
Host SpeciesMouse
FormulationTBS (pH7.4) containing 0.1% BSA and 0.05% NaN3
ReactivityDDK (FLAG tag): DYKDDDDK
Validated ApplicationIF, FC
Suggested DilutionFC: 1:50, IF: 1:50
Predicted Size
Storage/StabilityStore at -20ºC, stable for at least three months from date of shipment
Note This antibody doesn't recognize N- terminus DDK tag. Please use TA180144 for the detection of DDK tag at any location in Western blotting.
IF Image
CHO cells were tranfected with BAF57-Myc/DDK plasmid (OriGene Cat# RC209444). The cells were fixed with 2% formaldehyde for 20 min, and permeabilized with 0.1% saponin in PBS for 10 min. The cells were then incubated with DyLight488-labeled DDK antibodies diluted 1:50 in PBS/0.1% saponin for 20 min. The cells were then washed and observed under a fluorescent microscope. Please note the nucleus staining pattern for BAF57.
FC Image
HEK293T cells were transfected with PON3 cDNA (Cat# RC210019). After 48 hours, the cells were detached, fixed with 2% formaldehyde for 30 min, and permeabilized with 0.1% trixon-X100. The cells were then stained with DyLight488-conjugated mouse IgG1 (black line) or DyLight488- conjugated DDK antibodies diluted at 1:100 (red line).


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