OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
Home Antibody Antitag Antibodies

Anti-DDK Tag Antibody

div

Specifications Citations Related Products Product Documents
SKU Description Datasheet Price Availability  
TA150021 4C5, Anti-DDK monoclonal antibody, DyLight 488 , 100ul $318 In Stock Add to Shopping Cart

Specification

Product Name4C5, Anti-DDK monoclonal antibody, DyLight 488
Clone Nameclone 4C5
IsoTypeIgG2a
Concentration0.5mg/ml
ImmunogenAnti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide (DYKDDDDK) coupled to KLH
Host SpeciesMouse
FormulationTBS (pH7.4) containing 0.1% BSA and 0.05% NaN3
ReactivityDDK (FLAG tag): DYKDDDDK
Validated ApplicationIF, FC
Suggested DilutionFC: 1:50, IF: 1:50
Predicted Size
Storage/StabilityStore at -20ºC, stable for at least three months from date of shipment
IF Image
CHO cells were tranfected with BAF57-Myc/DDK plasmid (OriGene Cat# RC209444). The cells were fixed with 2% formaldehyde for 20 min, and permeabilized with 0.1% saponin in PBS for 10 min. The cells were then incubated with DyLight488-labeled DDK antibodies diluted 1:50 in PBS/0.1% saponin for 20 min. The cells were then washed and observed under a fluorescent microscope. Please note the nucleus staining pattern for BAF57.
FC Image
HEK293T cells were transfected with PON3 cDNA (Cat# RC210019). After 48 hours, the cells were detached, fixed with 2% formaldehyde for 30 min, and permeabilized with 0.1% trixon-X100. The cells were then stained with DyLight488-conjugated mouse IgG1 (black line) or DyLight488- conjugated DDK antibodies diluted at 1:100 (red line).

 

bar
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title