Anti-Myc (9E10) Monoclonal Antibody
Firgure 1: Immunoblot analysis of four different Myc-DDK tagged over-expression lysates and one Empty vector transfected 293T cells with TA150121 at 1:500
Catalog Number: TA150121
Product Name: Anti-Myc tag antibody
Description: Mouse monoclonal (IgG1) purified from mouse ascites fluids by affinity chromatography
Immunogen: Monoclonal antibody is produced by immunizing mice with AEEQKLISEEDLLRKRREQLKHKLE conjugated to KLH, corresponding to C terminal amino acids 408-432 of Human c-Myc.
Specificity/Sensitivity: The anti-Myc tag antibody recognizes recombinant proteins containing a Myc epitope tag fused to carboxy-termini proteins in transfected mammalian cells. The anti-Myc tag antibody (TA150121) also recognizes native c- Myc protein weighted 62KDa when it is diluted at 1:500.
Formulation: 0.64 mg/ml in PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide
Storage/Stability: Stable for 1 year at -20ºC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4ºC for 3 months.
Application: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows:Western Blot: 1:500-1:1000, Flow: 1:100, IF 1:100
Notes: This product is distributed for laboratory research use only, and is not intended for diagnostic use.
Firgure 2: HEK293T cells transfected with either Myc-DDK tagged PA2G4 plasmid (Red) or empty vector plasmid (Blue) were immunostained with anti-Myc mouse monoclonal antibody (TA150121) at 1:100, and then analyzed by flow cytometry.
Immunoprecipitation (IP) of Myc-DDK tagged HMGA2 and FCGR2A over expression lysate by
Firgure 4_1: Immunofluorescent staining of COS7 cells transiently transfected with Myc-DDK tagged MYD88 (RC202253).
Firgure 4_2: Immunofluorescent staining of COS7 cells transiently transfected with Myc-DDK tagged RASSF1 (RC213525).
Firgure 4_3: Immunofluorescent staining of COS7 cells transiently transfected with Myc-DDK tagged MEF2C (RC220584).