Firgure 1: Anti-V5 immunoblot analysis of six different HEK293T over-expression lysates containing different V5 tagged recombinant proteins at 1:2000 dilution.

Catalog Number: TA150094

Product Name: Anti-V5 tag antibody

Description: Mouse monoclonal antibody (IgG1) purified from mouse ascites fluids by affinity chromatography

Immunogen: Anti-V5 monoclonal antibody is produced by immunizing mice with a synthetic peptide (CGKPIPNPLLGLDSTR) coupled to KLH

Specificity/Sensitivity: The anti-V5 tag antibody recognizes recombinant proteins containing a V5 epitope tag fused carboxy-termini of targeted proteins in transfected mammalian cells expression systems.

Formulation: 0.92 mg/ml in PBS (pH 7.3) containing 1%BSA, 50% glycerol and 0.02% sodium azide

Storage/Stability: Stable for 1 year at -20ºC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4ºC for 3 months.

Application: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows: Western blot: 1:2000

Notes: This product is distributed for laboratory research use only, and is not intended for diagnostic use.