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Anti-ZYX Antibody EPR4302
Also for ZYX (NM_001010972)
|A synthetic peptide corresponding to residues in human Zyxin was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; FC: 1:100 - 1:500; ICC/IF: 1:100 - 1:250
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Rat
|Homo sapiens zyxin (ZYX), transcript variant 2|
|Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assembly. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways, while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.|
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Western blot - Zyxin antibody [EPR4302]; All lanes : Anti-Zyxin antibody [EPR4302] at 1/10000 dilution.Lane 1 : HeLa lysate.Lane 2 : MCF7 lysate.Lane 3 : C2C12 lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 61 kDa.Observed band size : 82 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Zyxin antibody [EPR4302]; Immunohistochemical analysis of Zyxin in paraffin embedded Human kidney tissue, using ab109316 at a 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Zyxin antibody [EPR4302]; Immunofluorescent staining of Zyxin in HeLa cells, using ab109316 at a 1/100 dilution.