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Anti-ZIC1 Antibody EPR7291(2)
Also for ZIC1 (NM_003412)
|A synthetic peptide corresponding to residues in human ZIC1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB: 1:1000 - 1:10000; ICC/IF: 1:100 - 1:250; FC: 1:100 - 1:1000
|Preservative: 0.01% Sodium azide|
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens Zic family member 1 (ZIC1)|
|ZIC1 is a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development. Aberrant expression of ZIC1 is seen in medulloblastoma, a childhood brain tumor. ZIC1 is closely linked to the zinc finger protein of the cerebellum 4. It is a transcription factor that can bind and transactivate apolipoprotein E (1). |
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Western blot - ; All lanes : Anti-Zic1 antibody [EPR7291(2)] at 1/1000 dilution.Lane 1 : Human cerebellum tissue lysate.Lane 2 : Human fetal brain tissue lysate.Lane 3 : SH SY5Y cell lysate.Lane 4 : SW480 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti rabbit at 1/2000 dilution.Predicted band size : 48 kDa.
Immunocytochemistry/ Immunofluorescence - ; Immunofluorescent staining of SW480 cells labelling Zic1 with ab134951 at 1/100 dilution
Flow Cytometry - Anti-Zic1 antibody [EPR7291(2)]; Overlay histogram showing SH-SY5Y cells stained with ab134951 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.