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Home Antibody All anti-YWHAZ antibodies

Anti-YWHAZ Antibody

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SKU Description Amount Price Availability*  
TA323870
  • Anti-YWHAZ Rabbit Polyclonal Antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC427673) , 20ug Explanation
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
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Also for YWHAZ (NM_001135699)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around aa.56~60 (R-S-S-W-R) derived from Human 14-3-3 zeta.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100
Predicted MW Explanation 28kDa
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta (YWHAZ), transcript variant 3
Alternative Name14-3-3-zeta; HEL-S-3; HEL4; KCIP-1; YWHAD
Database LinkNP_001129171
Entrez Gene 7534 Human
Entrez Gene 22631 Mouse
Entrez Gene 25578 Rat
FunctionThis gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.
Related Pathway Cell cycleOocyte meiosisNeurotrophin signaling pathwayPathogenic Escherichia coli infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 28 kDa . Positive control: HepG2, Hela and 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3: HepG2, Hela and 293 cells Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cytoplasm; Melanosome. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using YWHAZ (Ab-58) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)

 

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