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Anti-YWHAE Antibody EPR3919
Also for YWHAE (NM_006761)
|A synthetic peptide corresponding to residues in human 14-3-3E was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; FC: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC or IP.
|Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide (YWHAE), transcript variant 1|
|14-3-3E; KCIP-1; MDCR; MDS|
|The 14-3-3 epsilon protein (14-3-3E) is one of seven isoforms in the 14-3-3 protein family, which consists of acidic proteins expressed at high levels in neurons of the central nervous system (1). It is an adaptor protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3E binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (2). It also interacts with both insulin-like growth factor I receptor (IGFIR) and insulin receptor substrate I (IRS-1), suggesting its role in the regulation of insulin sensitivity (3). 14-3-3E has also been implicated in the pathogenesis of small cell lung cancer (4).|
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Western blot - 14-3-3 epsilon antibody [EPR3919]; All lanes : Anti-14-3-3 epsilon antibody [EPR3919].Lane 1 : Fetal brain lysate.Lane 2 : SW480 cell lysate.Lane 3 : NIH/3T3 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 29 kDa.
Immunocytochemistry/ Immunofluorescence - 14-3-3 epsilon antibody [EPR3919]; ab108210, at a 1/100 dilution, staining 14-3-3 epsilon in HeLa cells by Immunofluorescence.