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Home Antibody All anti-YWHAE antibodies

Anti-YWHAE Antibody EPR3918

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307143
  • Rabbit monoclonal antibody against 14-3-3E (clone EPR3918 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402021) , 20ug
100ul 325 In Stock
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WB(1)
IF(1)
FC(1)
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Also for YWHAE (NM_006761)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human 14-3-3E was used as an immunogen.
Clone NameEPR3918 IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsICC/IF: 1:100 - 1:250; WB: 1:1000 - 1:10000; FC: 1:10 - 1:1000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IHC-P or IP.

Reference Data

Target NameHomo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE), transcript variant 1
Alternative Name14-3-3E; HEL2; KCIP-1; MDCR; MDS
Database LinkNP_006752
FunctionThe 14-3-3 epsilon protein (14-3-3E) is one of seven isoforms in the 14-3-3 protein family; a family which consists of acidic 30 kDa proteins expressed at high levels in neurons of the central nervous system (1). It is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3E binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (2). It also interacts with both, the insulin-like growth factor I receptor (IGFIR) and the insulin receptor substrate I (IRS-1), suggesting its role in the regulation of insulin sensitivity (3). 14-3-3E has also been implicated in the pathogenesis of small cell lung cancer (4).
Related Pathway
Cell cycle

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WB Image
Western blot - 14-3-3 epsilon antibody [EPR3918]; All lanes : Anti-14-3-3 epsilon antibody [EPR3918] at 1/1000 dilution.Lane 1 : Human fetal brain cell lysate.Lane 2 : SH-SY5Y cell lysate.Lane 3 : 293T cell lysate.Lane 4 : SW480 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 29 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - 14-3-3 epsilon antibody [EPR3918]; TA307143 at 1/100 dilution staining 14-3-3 epsilon in HeLa cells by Immunofluorescence.
FC Image
Flow Cytometry - Anti-14-3-3 epsilon antibody [EPR3918]; Overlay histogram showing SH-SY5Y cells stained with TA307143 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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