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Anti-YBX1 GAT Antibody Polyclonal
UltraMAB Antibodies - Validated with 10K Protein Chip
Also for YBX1 (NM_004559)
|DNA immunization. This antibody was made against a protein fragment from the entire range of the protein (CAA65446).|
||WB: 1:100-1:2000; ELISA: 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody. (Protein A or G Sepharose)
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens Y box binding protein 1 (YBX1)|
|BP-8; CSDA2; CSDB; DBPB; MDR-NF1; NSEP-1; NSEP1; YB-1; YB1|
|The Y-box binding protein 1 (YB1) is a pluripotent DNA/RNA-binding factor which regulates gene expression through transcription and translation. YB1 has been shown to be a marker of tumor aggressiveness and belongs to the cold-shock domain family.|
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Western Blot: YB1 Antibody Western Blot was performed using affinity purified SEQer YB-1 antibody, aa(1-55) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer YB-1 antibody, aa(1-55) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.