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Home Antibody All anti-YBX1 antibodies

Anti-YBX1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA324830
  • Rabbit polyclonal YBX1 Antibody (C-term)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417905) , 20ug Explanation
400ul 325 3-7 Days
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WB(2)
IF(1)
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Also for YBX1 (NM_004559)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis YBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-305 amino acids from the C-terminal region of human YBX1.
Clone Name IsotypeIgG
Species ReactivityHuman (Predicted: Mouse, Rat, Bovine, Xenopus) Concentration2.0 mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:1000, IF: 1:10~50
Predicted MW Explanation 35924 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens Y box binding protein 1 (YBX1)
Alternative NameBP-8; CSDA2; CSDB; DBPB; MDR-NF1; NSEP-1; NSEP1; YB-1; YB1
Database LinkNP_004550
Entrez Gene 4904 Human
Entrez Gene 22608 Mouse
Entrez Gene 500538 Rat
Function
Related PathwayES Cell Differentiation/IPSTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of YBX1(arrow) using rabbit polyclonal YBX1 Antibody (C-term) (Cat. #TA324830). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YBX1 gene (Lane 2) (Origene Technologies).
WB Image
YBX1 Antibody (C-term) (Cat. #TA324830) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the YBX1 antibody detected the YBX1 protein (arrow).
IF Image
Fluorescent confocal image of U251 cell stained with YBX1 Antibody (C-term)(Cat#TA324830).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with YBX1 primary antibody (1:25, 1 h at 37â). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37â).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37â). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). YBX1 immunoreactivity is localized to Cytoplasm significantly.

 

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