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Home Antibody All anti-YAP1 antibodies

Anti-YAP1 Antibody EP1674Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300956
  • Rabbit Monoclonal Antibody against YAP1 (Clone EP1674Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401842) , 20ug Explanation
100uL 325 In Stock
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WB(2)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human YAP65 was used as an immunogen.
Clone NameEP1674Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:25000 - 1:50000; IP: 1:70; FC: 1:50; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 65 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens Yes-associated protein 1 (YAP1), transcript variant 2
Alternative NameCOB1; YAP; YAP2; YAP65; YKI
Database LinkNP_006097
Entrez Gene 10413 Human
FunctionThe transcriptional coactivator Yes-associated protein (YAP) has been shown to interact with and to enhance p73-dependent apoptosis in response to DNA damage. It has been shown that YAP requires the promyelocytic leukemia gene (PML) and nuclear body localization to coactivate p73. YAP imparts selectivity to p73 by promoting the activation of a subset of p53 and/or p73 target promoters (1). Akt promotes YAP localization to the cytoplasm, resulting in loss from the nucleus where it functions as a coactivator of transcription factors including p73. p73-mediated induction of Bax expression following DNA damage requires YAP function and is attenuated by Akt phosphorylation of YAP. YAP overexpression increases, while YAP depletion decreases, p73-mediated apoptosis following DNA damage, in an Akt inhibitable manner (2)
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - YAP1 antibody [EP1674Y]; Anti-YAP1 antibody [EP1674Y] at 1/50000 dilution + Hela cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 65 kDa.Observed band size : 65 kDa.
WB Image
Western blot - YAP1 antibody [EP1674Y]; .developed using the ECL technique.Predicted band size : 65 kDa.Observed band size : 70 kDa .Exposure time : 1 minute
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - YAP1 antibody [EP1674Y]; Immunohistochemical staining of YAP1 in paraffin embedded human breast carcinoma tissue using TA300956 at a 1/100 dilution.
FC Image
Flow Cytometry - YAP1 antibody [EP1674Y]; Overlay histogram showing HeLa cells stained with TA300956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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