Home Antibody All anti-XRCC6 antibodies
Also for XRCC6 (NM_001469)
|This XRCC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-548 amino acids from the C-terminal region of human XRCC6.|
|WB, IHC, IF, FC
||WB: 1:1000; IHC: 1:50-100; IF: 1:10-50; FC: 1:10-50
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
|This antibody is purified through a protein A column, followed by peptide affinity purification.
|Homo sapiens X-ray repair complementing defective repair in Chinese hamster cells 6 (XRCC6), transcript variant 1|
|CTC75; CTCBF; G22P1; KU70; ML8; TLAA|
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Western blot analysis of XRCC6 Antibody (C-term) in A2058 cell line lysates (35ug/lane).XRCC6 (arrow) was detected using the purified Pab.
Western blot analysis of XRCC6 (arrow) using rabbit polyclonal XRCC6 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XRCC6 gene.
XRCC6 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XRCC6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of XRCC6 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Fluorescent confocal image of Hela cell stained with XRCC6 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with XRCC6 primary antibody (1:25, 1 h at 37.). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37.).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37.). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). XRCC6 immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.
XRCC6 Antibody (C-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.