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Anti-XRCC6 Antibody EPR4026
Also for XRCC6 (NM_001469)
| A synthetic peptide corresponding to residues in human Ku70 was used as an immunogen.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:10000 - 1:50000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens X-ray repair complementing defective repair in Chinese hamster cells 6 (XRCC6), transcript variant 1|
|CTC75; CTCBF; G22P1; KU70; ML8; TLAA|
Entrez Gene 2547 Human
|ATP-dependent DNA helicase 2 subunit 1 (Ku70) is a single stranded DNA-dependent helicase originally recognized by the sera of patients with autoimmune diseases (1, 2). It plays a key role in multiple nuclear processes such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination (3). Ku70 forms a heterodimer with Ku80 and contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining (NHEJ) pathway (4). The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex, DNA-PK (1). Together, the Ku70/Ku80 heterodimer and DNA-PKcs are required for V(D)J recombination and DNA double-strand break repair and may also play a role in transcription regulation (5). In addition, the dimer associates with NARG1 to bind to the osteocalcin promoter and activate osteocalcin expression (1).|
|Transcription FactorsDruggable Genome Non-homologous end-joining|
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Western blot - Ku70 antibody [EPR4026]; All lanes : Anti-Ku70 antibody [EPR4026] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : Raji cell lysate.Lane 4 : A673 cell lysate.Lane 5 : A549 cell lysate.Lane 6 : A431 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 70 kDa.Observed band size : 70 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ku70 antibody [EPR4026]; TA307539, at 1/100 dilution, staining Ku70 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Ku70 antibody [EPR4026]; TA307539, at 1/100 dilution, staining Ku70 in HeLa cells by Immunofluorescence.