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Anti-XPO5 Antibody EPR8452
Also for XPO5 (NM_020750)
|A synthetic peptide corresponding to residues in human Exportin 5 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; FC: 1:10 - 1:100; ICC/IF: 1:50 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for IP.
|Homo sapiens exportin 5 (XPO5)|
Entrez Gene 57510 Human
Entrez Gene 72322 Mouse
Entrez Gene 363194 Rat
|Exportin 5 is a member of the karyopherin family that is required for the transport of small RNAs and double-stranded RNA-binding proteins from the nucleus to the cytoplasm. This protein translocates cargo through the nuclear pore complex in a RanGTP-dependent process (1). |
|Druggable Genome |
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Western blot - Anti-Exportin-5 antibody [EPR8452]; All lanes : Anti-Exportin-5 antibody [EPR8452] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : K562 cell lysate.Lane 3 : Human fetal heart lysate.Lane 4 : Human fetal brain lysate.Lane 5 : C6 cell lysate.Lane 6 : RAW264.7 cell lysate.Lane 7 : PC12 cell lysate.Lane 8 : NIH 3T3 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 136 kDa.Observed band size : 136 kDa.
Other-Anti-Exportin-5 antibody [EPR8452](TA311109); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Exportin-5 antibody [EPR8452]; TA311109, at 1/250 dilution, staining Exportin-5 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Flow Cytometry - Anti-Exportin-5 antibody [EPR8452]; Overlay histogram showing HEK293 cells stained with TA311109 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.