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Anti-WT1 Antibody

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SKU Description Amount Price Availability*  
TA323682
  • Anti-WT1 Rabbit Polyclonal Antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC424762) , 20ug
100ul $325 2-3 Weeks
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WB(1)

OriGene Data

ImmunogenFull length fusion protein
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration2.98mg/ml
Guaranteed Application *WB Suggested DilutionsWB: 1:500-2000
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens Wilms tumor 1 (WT1), transcript variant A
Alternative NameAWT1; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; WT33
Database LinkNP_000369
FunctionThis gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
Related Pathway

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WB Image
Predicted band size: 49 kDa . Positive control: Kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

 

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