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Anti-WIPF1 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for WIPF1 (NM_003387)
|DNA immunization. This antibody is specific for the Middle Region of the target protein.|
|Human , Mouse
|| WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens WAS/WASL interacting protein family, member 1 (WIPF1), transcript variant 1|
|PRPL-2; WASPIP; WIP|
|WIPF1 is involved in the organization of the actin cytoskeleton. The protein binds to a region of Wiskott-Aldrich syndrome protein that is frequently mutated in Wiskott-Aldrich syndrome, an X-linked recessive disorder. Impairment of the interaction between these WIPF1 and Wiskott-Aldrich syndrome protein may contribute to the disease. Two transcript variants encoding the same protein have been identified for this gene. WIPF1 binds to Wiskott-Aldrich syndrome protein, profilin and actin. It is involved with NCK1 and GRB2 in the recruitment and activation of N-WASP and may be involved in regulating the subcellular localization of N-WASP, resulting in the disassembly of stress fibers in filopodia formation.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WIPF1 (RC217601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WIPF1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-WIPF1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-WIPF1 rabbit polyclonal antibody. (TA590158)