Novel HIF2A mutations disrupt oxygen sensing, leading to polycythemia, paragangliomas, and somatostatinomas Blood, Mar 2013; 121: 2563 - 2566.
[anti-HA]
Regulation of the PI3-K/Akt Survival Pathway in the Rat Endometrium Biol Reprod, Mar 2013; 88: 79.
[Akt3]
RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP Nucleic Acids Res., Mar 2013; 10.1093/nar/gkt159.
[LA]
Ruxolitinib as potential targeted therapy for patients with JAK2 rearrangements Haematologica, Mar 2013; 98: 404 - 408.
[JAK2]
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-term region of human WIF1.
Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clone Name
Isotype
Rabbit Ig
Species Reactivity
Human
Concentration
0.25 mg/ml
Purification
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. (Protein A or G Sepharose)
Guaranteed Application *
WB, IHC
Suggested Dilutions
WB: 1:50~100; IHC: 1: 10~50; ELISA: 1: 1,000;
Background
WNT proteins are extracellular signaling molecules involved in the control of embryonic development. WIF1 is a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals.
Related Pathway
Wnt Signaling Pathway
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* OriGene provides validated application data and protocol, with money back guarantee.
Western blot analysis of WIF1 (arrow) using rabbit polyclonal WIF1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the WIF1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with WIF1 (Human C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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