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Anti-WAF1 Antibody WA-1
Also for WAF1 (NM_000389)
|This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.|
||ELISA: 1:5,000 - 1:20,000, WB: 1:2,000 - 1:10,000, IP: 1:100
|0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2|
|p21WAF1 is a tumor suppressor gene product critical to maintain proper regulatory control of cell division. p21WAF1 inhibits cyclin/cyclin-dependent kinase (cdk) complexes, causing cell cycle arrest by blocking cell division at the S phase. Various mutations of tumor suppressor genes have been associated with different types of cancers. In these cases, somatic gene therapy with wild-type versions of tumor suppressor genes have been contemplated as anti-cancer therapeutic approaches. p21WAF1 contains p53-binding sites in its promoter and expression of p21WAF1 is induced in WI38 fibroblasts with PMA. The induction of and
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Mab anti-Human p21WAF1 antibody (clone WA-1) is shown to detect human p21 by western blot. Detection occurs after 10 µg of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti-Human p21WAF1at room temperature for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:5,000. A single band corresponding to human p21WAF1 is detected at ~21 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p21WAF1. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.