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Home Antibody All anti-WAF1 antibodies

Anti-WAF1 Antibody WA-1

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA319554 Mouse monoclonal p21 WAF1 antibody 100ug $325 3-7 Days
LC409213 CDKN1A HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)

OriGene Data

ImmunogenThis protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.
Clone NameWA-1 Isotype
Species Reactivityhuman Concentration1.0 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:5,000 - 1:20,000, WB: 1:2,000 - 1:10,000, IP: 1:100
Buffer0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Note p21WAF1 is a tumor suppressor gene product critical to maintain proper regulatory control of cell division.  p21WAF1 inhibits cyclin/cyclin-dependent kinase (cdk) complexes, causing cell cycle arrest by blocking cell division at the S phase. Various mutations of tumor suppressor genes have been associated with different types of cancers.  In these cases, somatic gene therapy with wild-type versions of tumor suppressor genes have been contemplated as anti-cancer therapeutic approaches.  p21WAF1 contains p53-binding sites in its promoter and expression of p21WAF1 is induced in WI38 fibroblasts with PMA. The induction of and

Reference Data

Target NameHomo sapiens cyclin-dependent kinase inhibitor 1A (p21, Cip1) (CDKN1A), transcript variant 1
Alternative NameCAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1; SDI1; WAF1
Database LinkNP_000380
Entrez Gene 1026 Human
Function
Related PathwayDruggable Genome ErbB signaling pathwayCell cyclep53 signaling pathwayPathways in cancerGliomaProstate cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Mab anti-Human p21WAF1 antibody (clone WA-1) is shown to detect human p21 by western blot. Detection occurs after 10 µg of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti-Human p21WAF1at room temperature for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:5,000. A single band corresponding to human p21WAF1 is detected at ~21 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p21WAF1. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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