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Home Antibody All anti-VPS35 antibodies

Anti-VPS35 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA308721
  • Rabbit Polyclonal antibody to VPS35 (vacuolar protein sorting 35 homolog (S. cerevisiae))
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402657) , 20ug Explanation
100ul 325 3-7 Days
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WB(3)
IHC(1)
IF(1)

OriGene Data

ImmunogenSynthetic peptide corresponding to a region within amino acids 733 and 796 of VPS35 (Uniprot ID#Q96QK1)
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse (Predicted: Zebrafish, Chicken, Bovine) Concentration0.93mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
Predicted MW Explanation 92kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Zebrafish (86%), Chicken (93%), Bovine (100%)

Reference Data

Target NameHomo sapiens vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35)
Alternative NameMEM3; PARK17
Database LinkNP_060676
Entrez Gene 55737 Human
Entrez Gene 65114 Mouse
FunctionThis gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq]
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
VPS35 antibody detects VPS35 protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 7.5 % SDS-PAGE. VPS35 antibody (TA308721) dilution: 1:500
WB Image
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308721 diluted at 1:1000.
WB Image
Sample(30 ug of whole cell lysate). A:H1299. B:HeLa S3 . C:Hep G2 . D:MOLT4 . E:Raji . 7.5% SDS PAGE. TA308721 diluted at 1:500
IHC Image
VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term (TA308721) dilution: 1:250.
IF Image
Immunofluorescence analysis of methanol-fixed HCT116, using VPS35(TA308721) antibody at 1:500 dilution.

 

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