Home Antibody All anti-VPS35 antibodies
Also for VPS35 (NM_018206)
|Synthetic peptide corresponding to a region within amino acids 733 and 796 of VPS35 (Uniprot ID#Q96QK1)|
|Human, Mouse (Predicted: Zebrafish, Chicken, Bovine)
|WB, IHC, IF
||ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Purified by antigen-affinity chromatography.
|Seq homology of immunogen across species: Zebrafish (86%), Chicken (93%), Bovine (100%)
|Homo sapiens vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35)|
Entrez Gene 55737 Human
Entrez Gene 65114 Mouse
|This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq]|
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VPS35 antibody detects VPS35 protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 7.5 % SDS-PAGE. VPS35 antibody (TA308721) dilution: 1:500
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308721 diluted at 1:1000.
Sample(30 ug of whole cell lysate). A:H1299. B:HeLa S3 . C:Hep G2 . D:MOLT4 . E:Raji . 7.5% SDS PAGE. TA308721 diluted at 1:500
VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term (TA308721) dilution: 1:250.
Immunofluorescence analysis of methanol-fixed HCT116, using VPS35(TA308721) antibody at 1:500 dilution.