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Anti-VPS28 TrueMAB Antibody Clone 1A8
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for VPS28 (NM_016208)
|Full length human recombinant protein of human VPS28(NP_057292) produced in HEK293T cell.|
|Human , Dog , Rat , Monkey , Mouse
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
|| WB 1:200~2000, IHC 1:150, IF 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens vacuolar protein sorting 28 homolog (S. cerevisiae) (VPS28), transcript variant 1|
|This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally. [provided by RefSeq, Jul 2008]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VPS28 (RC200025, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VPS28.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-VPS28 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-VPS28 mouse monoclonal antibody. (TA505691)
Anti-VPS28 mouse monoclonal antibody (TA505691) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VPS28(RC200025).