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Anti-VIM TRUEMAB Antibody Clone 2F7
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Human recombinant protein fragment corresponding to amino acids 210-466 of human VIM (NP_003371) produced in E.coli.|
||WB 1:200 - 1:1000
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens vimentin (VIM)|
Entrez Gene 7431 Human
|This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]. |
|ES Cell Differentiation/IPS |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-VIM monoclonal antibody at 1:200.