The antiserum was produced against synthesized non-phosphopeptide derived from human Vimentin around the phosphorylation site of serine 56 (A-S-SP-P-G).
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clone Name
Isotype
IgG
Species Reactivity
Human
Concentration
1mg/ml
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
Guaranteed Application *
WB, IHC
Suggested Dilutions
WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:20000
Background
Vimentin (Ab-56) antibody detects endogenous levels of total Vimentin protein.
Related Pathway
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Western blot analysis of extracts from A549 cells, treated with Nocodazole (1ug/ml, 16hours), using Vimentin (Ab-56) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Vimentin (Ab-56) antibody.The picture on the right is treated with the synthesized peptide.
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