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Anti-VIM Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA309274
  • Chicken Anti-Vimentin Antibody
100ul $325 3-7 Days
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WB(1)
IF(1)

OriGene Data

ImmunogenRecombinant human vimentin purified from E. coli
Clone Name IsotypeIgY
Species Reactivityrat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:1,000, IF: 1:500
Buffertotal IgY fraction in PBS + 10 mM NaN3.
Purification Total IgY fraction

Reference Data

Target NameRattus norvegicus vimentin (Vim)
Alternative Name
Database LinkNP_112402
FunctionThis gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
Related Pathway

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WB Image
Western blot of NIH 3T3 cells showing specific immunolabeling of the ~50k Vimentin protein.
IF Image
Mixed neuron/glial cultures stained with anti-Vimentin (green) and rabbit anti-GFAP antibody (catalog #620-GFAP) (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.

 

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