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Anti-VEGFA Antibody EP1176Y
Also for VEGFA (NM_001025368)
|A synthetic peptide corresponding to residues on the C-terminus of human VEGF was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1000; IP: 1:50; FC: 1:100; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 4|
|MGC70609; MVCD1; VEGF; VPF|
|This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq]. |
Cytokine-cytokine receptor interaction
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Western blot - VEGF antibody [EP1176Y]; Anti-VEGF antibody [EP1176Y] at 1/1000 dilution + Recombinant protein (5ng).Secondary.Goat anti-Rabbit HRP labeled at 1/2000 dilution.Predicted band size : 27 kDa.Observed band size : 22 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF antibody [EP1176Y]; ab52917 stainingVEGF in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100and blocked with 5% serum for45 minutes at 25°C. Samples were incubated with primary antibody (1/400 in4% BSA + 5% serum in PBST) for 14 hours at 4°C. An Alexa Fluor? 546-conjugated Donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
Immunohistochemistry (Paraffin-embedded sections) - VEGF antibody [EP1176Y]; Ab52917 (1:50) staining human VEGF in human hemangioma tissue by immunohistochemistry using paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - VEGF antibody [EP1176Y]; Immunofluorescent staining of HUVEC cells using ab52917 (1:250).
Flow Cytometry - VEGF antibody [EP1176Y] - Carboxyterminal end; Overlay histogram showing HeLa cells stained with ab52917 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.