Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 6
Synonyms:
MVCD1; VEGF; VPF
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human VEGF1.
Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clone Name
Isotype
Rabbit Ig
Species Reactivity
Human
Concentration
0.25 mg/ml
Purification
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
Guaranteed Application *
WB, IHC
Suggested Dilutions
WB: 1: 100~500; IHC: 1:50~100; ELISA: 1: 1,000;
Background
VEGF1 is a member of the PDGF/VEGF growth factor family and and is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in VEGF1 have been associated with proliferative and nonproliferative diabetic retinopathy.
Related Pathway
Cytokine-cytokine receptor interaction
Focal Adhesion
Hemostasis
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Western blot analysis of VEGF1 Antibody (N-term) polyclonal antibody(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VEGF1 gene (Lane 2) (Origene Technologies). Molecular weight of monomeric VEGF1 is 27042 Da; higher observed molecular weight band is consistent with common form of disulfide linked homodimer.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with VEGF Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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