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Anti-VEGF TRUEMAB Antibody Clone OTI4E3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500289 VEGFA (VEGF) mouse monoclonal antibody, clone OTI4E3 (formerly 4E3) 100ul $325 In Stock
LC422435 VEGFA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500289 Carrier-free (BSA/glycerol-free) VEGF mouse monoclonal antibody, clone OTI4E3 (formerly 4E3) 100ug $450 3-4 weeks

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(2)
IHC(4)
IF(1)
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Also for VEGF (NM_001025366)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 27-233 of human VEGFA (NP_003367) produced in E.coli.
Clone NameClone OTI4E3 IsotypeIgG1
Species ReactivityHuman Concentration0.5 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:200 - 1:1000, IHC 1:50, IF 1:100,
Predicted MW Explanation 23.8 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1
Alternative NameMVCD1; VEGF; VPF
Database LinkNP_001020537
Entrez Gene 7422 Human
FunctionVEGF is a member of the PDGF/VEGF growth factor family that is often found as a disulfide linked homodimer. It is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in VEGF gene have been associated with proliferative and nonproliferative diabetic retinopathy.
Related PathwaySecreted ProteinDruggable Genome Cytokine-cytokine receptor interactionmTOR signaling pathwayVEGF signaling pathwayFocal adhesionPathways in cancerRenal cell carcinomaMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGF (RC223789, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGF.
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Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-VEGF monoclonal antibody.(1:200)
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500289)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500289)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500289)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VEGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500289)
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Anti-VEGF mouse monoclonal antibody (TA500289) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VEGF(RC223789).
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