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|Recombinant fragment corresponding to a region within amino acids 1 and 206 of VCP (Uniprot ID#P55072)|
|Human, Mouse (Predicted: Chicken, Pig, Xenopus laevis, Zebrafish, Bovine)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Purified by antigen-affinity chromatography.
|Seq homology of immunogen across species: Chicken (99%), Pig (99%), Xenopus laevis (98%), Zebrafish (97%), Bovine (99%), Xenopus tropicalis (99%)
|Homo sapiens valosin containing protein (VCP)|
|ALS14; HEL-220; HEL-S-70; IBMPFD; IBMPFD1; p97; TERA|
Entrez Gene 7415 Human
Entrez Gene 269523 Mouse
|The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]|
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VCP antibody detects VCP protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 7.5 % SDS-PAGE. VCP antibody (TA308570) dilution: 1:1000
Sample (50 ug of whole cell lysate). A: mouse brain. 7.5% SDS PAGE. TA308570 diluted at 1:5000.
Sample (30 ug of whole cell lysate). A: Hela. B: Hep G2 . C: Molt-4 . D: Raji. 7.5% SDS PAGE. TA308570 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using VCP(p97)(TA308570) antibody at 1:500 dilution.
VCP antibody [N1N2], N-term detects VCP protein at nucleus and cytosol on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. VCP antibody [N1N2], N-term (TA308570) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using VCP(p97)(TA308570) antibody at 1:200 dilution.