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Home Antibody All anti-VCP antibodies

Anti-VCP Antibody EPR3307(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307134
  • Rabbit monoclonal antibody against VCP(clone EPR3307(2))
  • FREE positive control: HEK293T cell transient overexpression lysate (LC402095) , 20ug
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for VCP (NM_007126)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human VCP was used as an immunogen.
Clone NameEPR3307(2) IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens valosin containing protein (VCP)
Alternative NameALS14; HEL-220; HEL-S-70; IBMPFD; IBMPFD1; p97; TERA
Database LinkNP_009057
FunctionVCP (Valosin-containing protein) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - VCP antibody [EPR3307(2)]; All lanes : Anti-VCP antibody [EPR3307(2)] at 1/10000 dilution.Lane 1 : MCF7 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : A549 cell lysate.Lane 4 : SH-SY5Y cell lysate.Lysates/proteins at 10 µg/ml per lane.Predicted band size : 89 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VCP antibody [EPR3307(2)]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using TA307134 at 1/100 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - VCP antibody [EPR3307(2)]; Immunofluorescent staining of HeLa cells using TA307134 at 1/100 dilution.
FC Image
Flow Cytometry-Anti-VCP antibody [EPR3307(2)](TA307134); Overlay histogram showing HL60 cells stained with TA307134 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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