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Anti-VCAN GAT Antibody Polyclonal
UltraMAB Antibodies - Validated with 10K Protein Chip
Also for VCAN (NM_004385)
|DNA immunization. This antibody is specific for the C Terminus Region of the target protein.|
|WB, ELISA, IHC
||WB: 1:500; ELISA: 1:100-1:2000; IHC: 1:3000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody. (Protein A or G Sepharose)
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens versican (VCAN), transcript variant 1|
|CSPG2; ERVR; GHAP; PG-M; WGN; WGN1|
|This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]|
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Western Blot: Versican Antibody - Western Blot was performed using affinity purified SEQer Versican antibody, aa(2521-2620) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer Versican antibody, aa(2521-2620) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Placenta; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunohistochemical staining of human oral mucosa shows distinct positivity in extracellular matrix. This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.