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Anti-VAV2 Antibody EP1067Y
Also for VAV2 (NM_003371)
|A synthetic peptide corresponding to residues on human Vav2 was used as an immunogen. This antibody detects Vav2 phosphorylated on Tyr172 as well as unphosphorylated Vav2.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IP
||WB: 1:20000; IP: Use at an assay dependent concentration; ICC: 1:100 - 1:250; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens vav 2 guanine nucleotide exchange factor (VAV2), transcript variant 2|
|VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
EGFR1 Signaling Pathway
Signaling by GPCR
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Western blot - VAV2 antibody [EP1067Y]; Anti-VAV2 antibody [EP1067Y] at 1/20000 dilution + 293 cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 101 kDa.Observed band size : 101 kDa.
Immunohistochemistry (Paraffin-embedded sections) - VAV2 antibody [EP1067Y]; Human cervical carcinoma stained with ab52640 at 1/50 - 1/100 dilution
Immunoprecipitation - Anti-VAV2 antibody [EP1067Y]; VAV2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Rabbit monoclonal to VAV2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). .The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52640.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 100kDa; VAV2.