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Anti-VAV2 Antibody EP1067Y
Also for VAV2 (NM_003371)
|A synthetic peptide corresponding to residues on human Vav2 was used as an immunogen. This antibody detects Vav2 phosphorylated on Tyr172 as well as unphosphorylated Vav2.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IP
||WB: 1:20000; IP: Use at an assay dependent concentration; ICC: 1:100 - 1:250; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt.
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Western blot - VAV2 antibody [EP1067Y]; Anti-VAV2 antibody [EP1067Y] at 1/20000 dilution + 293 cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 101 kDa.Observed band size : 101 kDa.
Immunohistochemistry (Paraffin-embedded sections) - VAV2 antibody [EP1067Y]; Human cervical carcinoma stained with TA303730 at 1/50 - 1/100 dilution
Immunoprecipitation - Anti-VAV2 antibody [EP1067Y]; VAV2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10ug of Rabbit monoclonal to VAV2 and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). .The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70oC; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with TA303730.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 100kDa; VAV2.