Anti-VASP Antibody, clone 1C3

Also for VASP (NM_003370)

OriGene Data

Immunogen	Full length human recombinant protein of human VASP (NP_003361) produced in HEK293T cell.
Clone Name	clone 1C3	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:500~2000, IF 1:100, FLOW 1:100
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens vasodilator-stimulated phosphoprotein (VASP)
Alternative Name	vasodilator-stimulated phosphoprotein
Database Link	NP_003361
Function	Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq].
Related Pathway	Focal Adhesion

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VASP (RC203544, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VASP.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-VASP monoclonal antibody.

IF Image

Anti-VASP mouse monoclonal antibody (TA502492) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VASP(RC203544).

FC Image

HEK293T cells transfected with either RC203544 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-VASP antibody(TA502492), and then analyzed by flow cytometry.

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Anti-VASP TrueMAB Antibody clone 1C3

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OriGene Data

Reference Data