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Home Antibody All anti-VASP antibodies

Anti-VASP TRUEMAB Antibody clone 1C3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA502492
  • VASP mouse monoclonal antibody, clone 1C3
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401150) , 20ug
100ul 325 In Stock
CF502492
  • Carrier-free (BSA/glycerol-free) VASP mouse monoclonal antibody, clone 1C3
100ug $450 3-4 weeks
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WB(2)
IF(1)
FC(1)
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Also for VASP (NM_003370)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human VASP (NP_003361) produced in HEK293T cell.
Clone Nameclone 1C3 IsotypeIgG1
Species ReactivityHuman Concentration0.45 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens vasodilator-stimulated phosphoprotein (VASP)
Alternative Namevasodilator-stimulated phosphoprotein
Database LinkNP_003361
FunctionVasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq].
Related Pathway
Focal Adhesion

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WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VASP (RC203544, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VASP.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-VASP monoclonal antibody.
IF Image
Anti-VASP mouse monoclonal antibody (TA502492) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VASP(RC203544).
FC Image
HEK293T cells transfected with either RC203544 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-VASP antibody(TA502492), and then analyzed by flow cytometry.

 

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