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Anti-VASP TRUEMAB Antibody clone 1H8
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for VASP (NM_003370)
|Full length human recombinant protein of human VASP (NP_003361) produced in HEK293T cell.|
|WB, IF, FC
||WB 1:500~2000, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens vasodilator-stimulated phosphoprotein (VASP)|
|Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VASP (RC203544, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VASP.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-VASP monoclonal antibody.
Anti-VASP mouse monoclonal antibody (TA502473) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VASP(RC203544).
HEK293T cells transfected with either RC203544 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-VASP antibody(TA502473), and then analyzed by flow cytometry.