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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VAPB (RC200517, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VAPB.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-VAPB polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-VAPB Rabbit Polyclonal antibody. (TA590191)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-VAPB Rabbit Polyclonal antibody. (TA590191)
Immunohistochemistry: VAPB Antibody - Immunohistochemical staining for vesicle-associated membrane protein B/C (VAPB) in human uterine corpus. Glandular cells stained strongly while cells in the endometrial stroma did not stain or stained weakly. This image is the courtesy of the Human Proteome Resource (HPR).
Immunohistochemistry: VAPB Antibody - Normal tissues generally showed strong cytoplasmic staining usually with membranous positivity. Malignant tissues displayed similar staining pattern as their normal counterparts. Image and statement courtesy of the Human Protein Atlas (HPA).
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-2 OS shows positivity in endoplasmic reticulum & golgi apparatus.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.