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Home Antibody All anti-USP7 antibodies

Anti-USP7 Antibody

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SKU Description Amount Price Availability*  
TA319169 Rabbit polyclonal anti-HAUSP antibody 100ug $325 3-7 Days
LC401171 USP7 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of human HAUSP protein.
Clone Name IsotypeIgG
Species Reactivityhuman Concentration1.0 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:15,000 - 1:60,000, WB: 1:500 - 1:2,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note HAUSP (also known as deubiquitinating enzyme 7, herpes virus associated ubiquitin specific protease, TEF1, ubiquitin carboxyl terminal hydrolase 7, ubiquitin specific protease 7, ubiquitin thiolesterase 7, and USP7) is a novel p53 interacting protein. HAUSP was identified by mass spectrometry of affinity purified p53 associated factors by Li et al. HAUSP strongly stabilizes p53, even in the presence of excess MDM2, and also induces p53-dependent cell growth repression and apoptosis. HAUSP has an intrinsic enzymatic activity that specifically de-ubiquitinates p53 both in vivo and in vitro. Expression of a catalytically inactive point mutation of HAUSP in cells increased the levels of p53 ubiquitination and also destabilized p53. Li et al concluded that their findings revealed an important mechanism by which p53 can be stabilized by direct de-ubiquitination and also implied that HAUSP may function as a tumor suppressor in vivo through the stabilization of p53.

Reference Data

Target NameHomo sapiens ubiquitin specific peptidase 7 (herpes virus-associated) (USP7), transcript variant 1
Alternative NameHAUSP; TEF1
Database LinkNP_003461
Entrez Gene 7874 Human
Function
Related PathwayProteaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot using affinity purified anti-HAUSP antibody shows detection of HAUSP in various cell lysates at 130 kDa (lane 1 - HeLa nuclear extract, lane 2 - HeLa, Lane 3 - A431, Lane 4 - MCF7, Lane 5 - 3T3).  The antibody is blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates.  A 1:500 dilution of the primary antibody was used for detection followed by a 1:5,000 dilution of HRP Gt-a-Rabbit IgG.  Exposure time was 30 s.
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