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Home Antibody All anti-USP5 antibodies

Anti-USP5 TRUEMAB Antibody Clone OTI2F5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA501288 anti-USP5 mouse monoclonal antibody, clone OTI2F5 (formerly 2F5) 100ul $325 In Stock
LC418652 USP5 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501288 Carrier-free (BSA/glycerol-free) USP5 mouse monoclonal antibody, clone OTI2F5 (formerly 2F5) 100ug $450 3-4 weeks
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WB(2)
IHC(4)
IF(1)

OriGene Data

ImmunogenFull length human recombinant protein of human USP5(NP_003472) produced in HEK293T cell.
Clone NameClone OTI2F5 IsotypeIgG1
Species ReactivityHuman Concentration0.67 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:200 - 1:1000, IHC 1:50, IF 1:100,
Predicted MW Explanation 93.1 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 2
Alternative NameISOT
Database LinkNP_003472
Entrez Gene 8078 Human
FunctionUbiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM].
Related PathwayProteaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP5 (RC202624, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP5.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-USP5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501288, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501288, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501288, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501288, Dilution 1:50)
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Anti-USP5 mouse monoclonal antibody (TA501288) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY USP5(RC202624).
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