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Home Antibody All anti-USP5 antibodies

Anti-USP5 TRUEMAB Antibody clone 2F5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501288
  • anti-USP5 mouse monoclonal antibody, clone 2F5
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418652) , 20ug Explanation
100ul 325 In Stock
CF501288
  • Carrier-free (BSA/glycerol-free) USP5 mouse monoclonal antibody, clone 2F5
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(1)
IHC(4)
IF(1)
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Also for USP5 (NM_003481)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human USP5(NP_003472) produced in HEK293T cell.
Clone Nameclone 2F5 IsotypeIgG1
Species ReactivityHuman Concentration0.67 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100,
Predicted MW Explanation 93.1 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 2
Alternative NameISOT
Database LinkNP_003472
Entrez Gene 8078 Human
FunctionUbiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM].
Related PathwayProteaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP5 (RC202624, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP5.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501288, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501288, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501288, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501288, Dilution 1:50)
IF Image
Anti-USP5 mouse monoclonal antibody (TA501288) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY USP5(RC202624).

 

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