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Home Antibody All anti-USP5 antibodies

Anti-USP5 TRUEMAB Antibody clone 1F8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501287
  • anti-USP5 mouse monoclonal antibody, clone 1F8
  • FREE positive control: HEK293T cell transient overexpression lysate (LC418652) , 20ug
100ul 325 In Stock
CF501287
  • Carrier-free (BSA/glycerol-free) USP5 mouse monoclonal antibody, clone 1F8
100ug $450 3-4 weeks
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WB(2)
IHC(2)
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Also for USP5 (NM_003481)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human USP5 (NP_003472) produced in HEK293T cell.
Clone Nameclone 1F8 IsotypeIgG2a
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.70 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB 1:1000~2000, IHC 1:50,
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 2
Alternative NameISOT
Database LinkNP_003472
FunctionUbiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP5 (RC202624, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP5.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-USP5 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501287, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501287, Dilution 1:50)

 

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