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Anti-USP5 TRUEMAB Antibody clone 1F8
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for USP5 (NM_003481)
|Full length human recombinant protein of human USP5 (NP_003472) produced in HEK293T cell.|
|Human, Dog, Rat, Monkey
||WB 1:1000~2000, IHC 1:50,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 2|
Entrez Gene 8078 Human
Entrez Gene 297593 Rat
Entrez Gene 714032 Monkey
Entrez Gene 486718 Dog
|Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP5 (RC202624, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP5.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-USP5 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501287, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-USP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501287, Dilution 1:50)