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Anti-USP18 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319486
  • Rabbit polyclonal anti-UBP43 antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC413772) , 20ug
100ug $325 3-7 Days
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OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 40-49 of human UBP43 protein.
Clone Name IsotypeIgG
Species Reactivityhuman Concentration0.9 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:10,000 - 1:50,000, WB: 1:500 - 1:2,000, IF: User Optimized
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note UBP43 (also known as ISG43, Ubl thiolesterase 18, ISG15-specific processing protease, 43 kDa ISG15-specific protease, ubiquitin specific protease 18 or USP18) is a member of the deubiquitinating protease family of enzymes, which removes ubiquitin adducts from a broad range of protein substrates. Deletion of the UBP43 gene in mouse leads to a massive increase of ISG15 conjugates in tissues indicating that UBP43 is a major ISG15-specific protease. UBP43 is the first ISG15-specific protease reported.  Both ISG15 and UBP43 genes are known to be strongly induced by interferon, genotoxic stress, and viral infection. Thus UBP43 may be necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.

Reference Data

Target NameHomo sapiens ubiquitin specific peptidase 18 (USP18)
Alternative NameISG43; UBP43
Database LinkNP_059110
Function
Related Pathway

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WB Image
Western blot using Affinity Purified anti-UBP43 antibody shows detection of a band ~43 kDa band (arrowhead) believed to be to UBP43 in lysates from HeLa nuclear extracts (lane 1) and whole cell lysates from HeLa (lane 2), A431 (lane 3, Jurkat (Lane 4) and HEK293 (lane 5).  Approximately 18 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-UBP43 antibody. Signal was detected using standard techniques. The strong band between the 37kD and 50kD markers is expected to be UBP43. The other bands are probably non-specific.

 

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