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Anti-USP13 Antibody EPR4348
Also for USP13 (NM_003940)
|A synthetic peptide corresponding to human USP13 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; ICC: 1:100 - 1:250; FC: 1:100 - 1:500; ICC/IF: Use a concentration of 10 ug/ml
|Does not react with Rat. Is unsuitable for IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IHC-P.
|Homo sapiens ubiquitin specific peptidase 13 (isopeptidase T-3) (USP13)|
|USP13 (Ubiquitin carboxyl-terminal hydrolase 13) is involved in thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a 76-residue protein attached to proteins as an intracellular targeting signal (1). It is highly expressed in the ovary and testes (1).|
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Western blot - USP13 antibody [EPR4348]; All lanes : Anti-USP13 antibody [EPR4348] at 1/1000 dilution.Lane 1 : SH SY5Y cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : A375 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 97 kDa.Observed band size : 97 kDa.
Immunocytochemistry/ Immunofluorescence - Anti-USP13 antibody [EPR4348]; ICC/IF image of ab109264 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109264 at 10ug/ml overnight at +4°C. The secondary antibody (green) was DyLight? 488 goat anti- rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.