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UltraMAB

ABAT UltraMAB Antibody, Clone UMAB179

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
UM800071
  • ABAT mouse monoclonal antibody,clone UMAB179, 100 ul
$468 In Stock Add to Shopping Cart
UM870071
  • ABAT mouse monoclonal antibody,clone UMAB179, 30 ul
$120 In Stock Add to Shopping Cart
UM800071CF
  • Carrier-free (BSA/glycerol-free) ABAT mouse monoclonal antibody,clone UMAB179
$528 3 days Add to Shopping Cart
WB(2)
Data Img
IHC(5)
Data Img
IF(1)
Data Img
10K-CHIP(1)
Data Img

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 29-323 of human ABAT(NP_065737) produced in E.coli.
Clone NameClone UMAB179 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested Dilutions IHC 1:100~400,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from cell culture supernatant by affinity chromatography

Reference Data

Target NameHomo sapiens 4-aminobutyrate aminotransferase (ABAT), transcript variant 1
Alternative NameGABA-AT; GABAT; NPD009
Database LinkNP_065737
Function4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008].
Related Pathway

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Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).
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Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-ABAT monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen).(1:500) Dilution: 1:500
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Immunohistochemical staining of paraffin-embedded human kidney tissue using anti-ABAT mouse monoclonal antibody. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) in pressure chamber/cooker at 110C for 3 min, UM800071@ 1:400 shows kidney tubules with strong granular cytoplasmic staining.
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Immunohistochemical staining of paraffin-embedded carcinoma of human liver tissue using anti-ABAT mouse monoclonal antibody.(Heat-induced epitope retrieval in pressure chamber/cooker at 110C for 3 min, UM800071(1:400). Images shows cancer cells with strong granular cytoplasmic staining.
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Immunohistochemical staining of paraffin-embedded human pancreas tissue using anti-ABAT mouse monoclonal antibody. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) in pressure chamber/cooker at 110C for 3 min, UM800071(1:400). Images shows exocrine granular cells with strong granular cytoplasmic staining.
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Immunohistochemical staining of paraffin-embedded carcinoma of human thyroid tissue using ABAT clone UMAB179, mouse monoclonal antibody. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0 ) in pressure chamber/cooker at 110C for 3 min, UM800071 was diluted 1:1000 using HRP detection and DAB chromogen. Image shows strong cytoplasmic and membranous staining is present in the tumor cells.
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Immunohistochemical staining of paraffin-embedded human liver using ABAT clone UMAB179, mouse monoclonal antibody at 1:400 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800071 requires heat-induced epitope retrieval with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong cytoplasmic and membranous staining of the hepatocytes no staining in the bile duct.
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Immunofluorescent staining of HepG2 cells using anti-ABAT mouse monoclonal antibody (UM800071, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).
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OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ABAT mouse monoclonal antibody (UM800071). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)
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