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PGR UltraMAB Antibody, Clone UMAB136
UltraMAB Antibodies - Validated with 10K Protein Chip
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|Homo sapiens progesterone receptor (PGR), transcript variant 2|
|Human recombinant protein fragment corresponding to amino acids 1-298 of human PGR (NP_000917) produced in E.coli.|
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
||0.5~1.0 mg/ml (Lot Dependent)
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|WB, IHC, IF
|| WB 1:2000, IHC 1:200,
|This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Jan 2011]. |
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Western blot analysis of MCF7 cell lysate (35ug) by using anti-PGR monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PGR mouse monoclonal antibody. (UM800037; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
Immunofluorescent staining of MCF-7 cells using anti-PGR mouse monoclonal antibody (UM800037, green, 1:50). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.