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CD2 UltraMAB Antibody, Clone UMAB86
UltraMAB Antibodies - Validated with 10K Protein Chip
|Full length human recombinant protein of human CD2 (NP_001758) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|10K-CHIP, WB, IHC, IF
|| IHC 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens CD2 molecule (CD2), transcript variant 2|
|LFA-2; SRBC; T11|
|CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells (summarized by Sewell et al., 1986 [PubMed 3490670]). It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it.[supplied by OMIM, Jul 2010]. |
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OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CD2 mouse monoclonal antibody (UM800017). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
Western Blot analysis of extracts (35µg) from 10 different cell lines by using anti-CD2 monoclonal antibody (Clone UMAB86)
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD2 mouse monoclonal antibody. (UM800017; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD2 mouse monoclonal antibody. (UM800017; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.
Immunohistochemical staining of paraffin-embedded human spleen using anti-CD2 mouse monoclonal antibody at 1: 200 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800017 requires HIER with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong cytoplamic and membrane staining.
Immunohistochemical staining of paraffin-embedded human melanoma using anti-CD2 mouse monoclonal antibody at 1: 200 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800017 requires HIER with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong cytoplamic and membrane staining infiltrating T cells but no staining in the tumor cells..
Immunofluorescent staining of Jurkat cells using anti-CD2 mouse monoclonal antibody (UM800017, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.