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CD20 UltraMAB Antibody, Clone UMAB58
UltraMAB Antibodies - Validated with 10K Protein Chip
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Also for CD20 (NM_021950)
|Full length human recombinant protein of human MS4A1 (NP_068769) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, 10K-CHIP
|| IHC 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens membrane spanning 4-domains A1 (MS4A1), transcript variant 3|
|B1; Bp35; CD20; CVID5; LEU-16; MS4A2; S7|
|This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul |
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Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:250.
Immunohistochemical staining of paraffin-embedded human spleen using anti-CD20 clone UMAB58 mouse monoclonal antibody at 1:200 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800009 requires heat-induced epitope retrieval withCitrate pH6.0 for 3minutes at110C in pressure chamber . The image shows the cells in the white pulp have strong cytoplasmic and membraneous staining.
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-MS4A1 mouse monoclonal antibody. (UM800009; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MS4A1 mouse monoclonal antibody. (UM800009; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescent staining of Jurkat cells using anti-CD20 mouse monoclonal antibody (UM800009, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 4µm.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-MS4A1 mouse monoclonal antibody (UM800009). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.