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UltraMAB

CD146 UltraMAB Antibody, Clone UMAB154

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
UM800051
  • CD146 (MCAM) mouse monoclonal antibody,clone UMAB154, 100 ul
$468 In Stock Add to Shopping Cart
UM870051
  • CD146 (MCAM) mouse monoclonal antibody,clone UMAB154, 30 ul
$120 In Stock Add to Shopping Cart
WB(5)
Data Img
IHC(15)
Data Img
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Also for CD146 (NM_006500)
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OriGene Data

ImmunogenFull length human recombinant protein of human MCAM (NP_006491) produced in HEK293T cell.
Clone NameClone UMAB154 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested Dilutions WB 1:2000, IHC 1:200,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens melanoma cell adhesion molecule (MCAM)
Alternative NameCD146; MUC18
Database LinkNP_006491
Function
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.
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Western blot analysis of MALME3M cell lysate (35ug) by using anti-MCAM monoclonal antibody.
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Western blot analysis of  Hela cell lysate (35ug) by using anti-MCAM monoclonal antibody.
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Western blot analysis of  HEK293 cell lysate (35ug) by using anti-MCAM monoclonal antibody.
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Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500
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Immunohistochemical staining of paraffin-embedded human melanoma using anti- CD146 (MCAM) clone UMAB154 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800051 requires HIER with with Accel 3in1 EDTA solution ph8.7 at 110C for 3 min using pressure chamber/cooker. The tumor cells shows strong membraneous and cytoplasmic staining.
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Immunohistochemical staining of paraffin-embedded Human tonsil using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-MCAMMCAM monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MCAMMCAM monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
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