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UltraMAB

DOCK2 UltraMAB Antibody, Clone UMAB142

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
UM800039
  • DOCK2 mouse monoclonal antibody,clone UMAB142, 100 ul
$468 In Stock Add to Shopping Cart
UM870039
  • DOCK2 mouse monoclonal antibody,clone UMAB142, 30 ul
$120 In Stock Add to Shopping Cart
UM800039CF
  • Carrier-free (BSA/glycerol-free) DOCK2 mouse monoclonal antibody,clone UMAB142
$528 3 days Add to Shopping Cart
WB(2)
Data Img
IHC(4)
Data Img
IF(1)
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OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 1544-1830 of human DOCK2 (NP_004937) produced in E.coli.
Clone NameClone UMAB142 IsotypeIgG2a
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested Dilutions WB 1:2000, IHC 1:150,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens dedicator of cytokinesis 2 (DOCK2)
Alternative NameFLJ46592; KIAA0209
Database LinkNP_004937
FunctionThe protein encoded by this gene belongs to the CDM protein family. It is specifically expressed in hematopoietic cells, predominantly in the peripheral blood leukocytes, and is involved in remodeling of the actin cytoskeleton required for lymphocyte migration, through the activation of RAC. Mice lacking this gene show a severe impairment in the migration and homing of lymphocytes. These mutant mice also exhibited long-term survival of allografts, suggesting that this gene may be a target for controlling transplant rejection. [provided by RefSeq, Oct 2011].
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of  Jurkat cell lysate (35ug) by using anti-DOCK2 monoclonal antibody.
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Western blot analysis of RPMI8226 cell lysate (35ug) by using anti-DOCK2 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DOCK2 mouse monoclonal antibody. (UM800039; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded human lung cancer using anti-DOCK2 clone UMAB142 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800039 requires HIER with with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. IHC staining shows tumor cells are negative however infiltrating lymphocytes strongly positive.
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Immunohistochemical staining of paraffin-embedded human melanoma using anti-DOCK2 clone UMAB142 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800039 requires HIER with with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. IHC staining shows tumor cells are negative however infiltrating lymphocytes strongly positive.
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Immunohistochemical staining of paraffin-embedded human tonsil using anti-DOCK2 clone UMAB142 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800039 requires HIER with with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. IHC staining shows germinal and nongerminal center with strong stain in the cytoplasm and membrane.
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Immunofluorescent staining of Jurkat cells using anti-DOCK2 mouse monoclonal antibody (UM800039, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.
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