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PGR UltraMAB Antibody, Clone UMAB135
UltraMAB Antibodies - Validated with 10K Protein Chip
|Human recombinant protein fragment corresponding to amino acids 1-298 of human PGR (NP_000917) produced in E.coli.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
|| IHC 1:200,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens progesterone receptor (PGR), transcript variant 2|
|This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce two isoforms, A and B. The two isoforms are identical except for the additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap. [provided by RefSeq, Jan 2011]. |
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Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-PGR mouse monoclonal antibody. (UM800036; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PGRPGRmonoclonal antibody. (UM800036; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min)
Immunofluorescent staining of MCF-7 cells using anti-PGR mouse monoclonal antibody (UM800036, green, 1:50). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.